Post-Transcriptional Regulatory Crosstalk between MicroRNAs and Canonical TGF-ß/BMP Signalling Cascades on Osteoblast Lineage: A Comprehensive Review.

MicroRNAs (miRNAs) are a family of small, single-stranded, and non-protein coding RNAs about 19 to 22 nucleotides in length, that have been reported to have important roles in the control of bone development. MiRNAs have a strong influence on osteoblast differentiation through stages of lineage commitment and maturation, as well as via controlling the activities of osteogenic signal transduction pathways. Generally, miRNAs may modulate cell stemness, proliferation, differentiation, and apoptosis by binding the 3'-untranslated regions (3'-UTRs) of the target genes, which then can subsequently undergo messenger RNA (mRNA) degradation or protein translational repression. MiRNAs manage the gene expression in osteogenic differentiation by regulating multiple signalling cascades and essential transcription factors, including the transforming growth factor-beta (TGF-ß)/bone morphogenic protein (BMP), Wingless/Int-1(Wnt)/ß-catenin, Notch, and Hedgehog signalling pathways; the Runt-related transcription factor 2 (RUNX2); and osterix (Osx). This shows that miRNAs are essential in regulating diverse osteoblast cell functions. TGF-ßs and BMPs transduce signals and exert diverse functions in osteoblastogenesis, skeletal development and bone formation, bone homeostasis, and diseases. Herein, we highlighted the current state of in vitro and in vivo research describing miRNA regulation on the canonical TGF-ß/BMP signalling, their effects on osteoblast linage, and understand their mechanism of action for the development of possible therapeutics. In this review, particular attention and comprehensive database searches are focused on related works published between the years 2000 to 2022, using the resources from PubMed, Google Scholar, Scopus, and Web of Science.

Self-Pulsations in Terahertz Quantum Cascade Lasers under Strong Optical Feedback: The Effect of Multiple Reflections in the External Cavity.

We have recently reported the self-pulsation phenomenon under strong optical feedback in terahertz (THz) quantum cascade lasers (QCLs). One important issue, however, we left open: the effect of multiple round trips in the external cavity on the laser response to feedback. Our current analysis also casts additional light on the phenomenon of self-pulsations. Using only one external cavity round trip (ECRT) in the model has been the common approach following the seminal paper by Lang-Kobayashi in 1980. However, the conditions under which the Lang-Kobayashi model, in its original single-ECRT formulation, is applicable has been rarely explored. In this work, we investigate the self-pulsation phenomenon under multiple ECRTs. We found that the self-pulsation waveform changes when considering more than one ECRT. This we attribute to the combined effect of the extended external cavity length and the frequency modulation of the pulsation frequency by the optical feedback. Our findings add to the understanding of the optical feedback dynamics under multiple ECRTs and provide a pathway for selecting the appropriate numerical model to study the optical feedback dynamics in THz QCLs and semiconductor lasers in general.

The Regulatory Role of MicroRNAs in Breast Cancer.

MicroRNAs (miRNAs) are small non-coding RNA molecules which function as critical post-transcriptional gene regulators of various biological functions. Generally, miRNAs negatively regulate gene expression by binding to their selective messenger RNAs (mRNAs), thereby leading to either mRNA degradation or translational repression, depending on the degree of complementarity with target mRNA sequences. Aberrant expression of these miRNAs has been linked etiologically with various human diseases including breast cancer. Different cellular pathways of breast cancer development such as cell proliferation, apoptotic response, metastasis, cancer recurrence and chemoresistance are regulated by either the oncogenic miRNA (oncomiR) or tumor suppressor miRNA (tsmiR). In this review, we highlight the current state of research into miRNA involved in breast cancer, with particular attention to articles published between the years 2000 to 2019, using detailed searches of the databases PubMed, Google Scholar, and Scopus. The post-transcriptional gene regulatory roles of various dysregulated miRNAs in breast cancer and their potential as therapeutic targets are also discussed.